QuickTip for Plasma Separation – Separate plasma from blood in minutes without the need for centrifugation

Plasma separation is passively achieved.  The monolith is a dry substrate and when it comes in contact with liquid, the liquid is absorbed, much like a sponge.  White blood cells are captured and remain isolated at the point where blood is introduced onto the monolith; red blood cells are isolated next, and plasma continues to migrate past the RBCs, eventually saturating the monolith.  This occurs in minutes, not hours.

White blood cells are not lysed; gDNA remains isolated.

Red blood cells are not lysed and remain intact, preventing interference with downstream assays.

Advantages

  • Separate plasma from blood without the need for centrifugation, vacuum, or other instrumentation
  • Real Time PCR ready DNA in about 2 minutes (depending on input volume)
  • Small particles (e.g., bacteria, viruses) are eluted with minimal positive pressure
  • Suitable for low and medium throughput applications
  • Plasma eluate can be added directly into amplification mix
  • Low cost:  about $2-$2.50 per sample depending on size

Applications

  • cfDNA Capture
  • Point-of-Care screening – easily incorporated into cartridge type assays
  • Infectious disease testing (e.g., viruses, fungi, bacteria, unicellular parasites)
  • QuickTip for Plasma is for Research Use Only – not intended for clinical diagnostics

Real Time PCR ready analysis of DNA in plasma in two easy steps in two minutes.  Ideal for low to medium throughput environments and easily adapted for automated processing.  The Plasma QuickTip can be manufactured in virtually any shape and size, making it versatile and easy to adapt to your customized consumable.

There is no need for expensive instrumentation, centrifuges, or magnets. Simply dispense blood onto the substrate and walk away.  Return in 2-3 minutes and elute the plasma.

See how it works with 600 uL of blood (about 3 1/2 mins)

QuickTip for White Blood Cells – Isolate white blood cells in minutes

The WBC QuickTip is essentially the Plasma QuickTip used in reverse order.  Instead of dispensing a blood sample onto the monolith, the QuickTip is inserted into the blood sample.  WBC isolation is passively achieved.  The monolith is a dry substrate and when it comes in contact with liquid, the liquid is absorbed.  White blood cells are captured and remain isolated at the point where blood meets the monolith.  Again, this occurs in minutes, not hours.

White blood cells are not lysed; gDNA remains isolated.  About a 1mm section of the monolith (the part with the WBCs in it) is excised and added directly into a PCR or LAMP reaction.  The monolith material does not inhibit the amplification reaction.

Advantages

  • Isolate white blood cells without the need for enzymatic incubation, centrifugation, vacuum, or other instrumentation
  • Real Time PCR ready gDNA in less than 5 minutes
  • No need to elute DNA – saving time and handling
  • Suitable for low and medium throughput applications
  • gDNA is captured in monolith media
  • Monolith with gDNA can be inserted directly into amplification mix
  • Low cost:  about $1.50 per sample

Applications

  • Rapid genotyping for pharmacogenomics
  • Clinical research
  • Hereditary analysis
  • Identity testing

White blood cells are isolated in a few easy steps.  Ideal for low to medium throughput environments and easily adapted for automated processing.  Video shows use of reusable cutting tool.  Washing and sterilization of cutter and manifold elimination cross contamination of samples.